What Is An HFR Strain?

A high-frequency recombination cell (Hfr cell) (also called an Hfr strain) is a bacterium with a conjugative plasmid (for example, the F-factor) integrated into its chromosomal DNA. The integration of the plasmid into the cell’s chromosome is through homologous recombination.

What is the difference between f+ and hfr?

The key difference between F+ strains and Hfr is that F+ strains have F plasmids in the cytoplasm freely without integrating into bacterial chromosomes while Hfr strains have F plasmids integrated to their chromosomes.

what is an HFR cell quizlet? Hfr cell is an F+ cell, where the F factor is integrated in the chromosome (plasmid becomes a episome)

what causes a bacterial strain to be designated HFR?

All Hfr strains arise from the integration of a conjugative plasmid into the bacterial chromosome by one of several possible types of recombination events. The most commonly used Hfr strains have been formed by either spontaneous or UV-induced integration of the E. coli F factor (see chapter 126).

What is interrupted mating?

interrupted mating. A technique used to MAP bacterial genes by determining the sequence in which donor genes enter recipient Cells. A gene MAPping technique in which bacterial conjugation is disrupted after specified time intervals.

What does HFR mean?

higher frame rate

What is an F+?

Noun. F+ An academic grade given by certain institutions. Slightly better than an F and slightly worse than an E- (or, in most of the US, a D-).

What is the purpose of conjugation?

Conjugation is the process by which one bacterium transfers genetic material to another through direct contact. During conjugation, one bacterium serves as the donor of the genetic material, and the other serves as the recipient. The donor bacterium carries a DNA sequence called the fertility factor, or F-factor.

What does the F plasmid do?

F plasmid. The F plasmid is an example of a large plasmid, which contains genes that allow the plasmids DNA to be transferred between cells. It is found in the bacterium E. This joining via a pilus in order to transfer DNA between bacteria is known as conjugation.

What is an F factor in conjugation?

noun Genetics. Also called F factor, fertility factor. a plasmid in certain bacteria that enables transfer of genetic material from a donor cell to a recipient by conjugation, resulting in recombination.

Can f+ conjugate with F+?

The bacterium becomes F-, and can only act as the recipient. (No, the bacterium still has the F factor, so it cannot be the recipient.) The bacterium is F+, and is the donor. When the F factor is integrated into the bacterial chromosome, it can still act as the donor in a conjugation cross.

What is f prime in genetics?

F’ (F-prime) bacteria are formed by incorrect excision from the chromosome, resulting in F plasmid carrying bacterial sequences that are next to where the F episome has been inserted. F− bacteria do not contain F factor and act as the recipients.

How do Episomes integrate into chromosomes?

They are also designed to integrate into the chromosome following their movement from one cell to another. Like plasmids, transposons can carry other genetic material with them, and so pass on resistance to the cells they enter.

How is a Merozygote formed?

How is a merozygote formed? The F factor and several adjacent genes are excised from the chromosome of an F+ cell and transferred to an F− strain; A merozygote is formed when the F factor and several adjacent bacterial genes are excised from the bacterial chromosome of an F′ cell and transferred to an F− cell.

What is bacterial plasmid?

A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell’s chromosomal DNA. Plasmids naturally exist in bacterial cells, and they also occur in some eukaryotes. Often, the genes carried in plasmids provide bacteria with genetic advantages, such as antibiotic resistance.

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